Japanese Pear Transcriptome Database
Japanese pear (P. pyrifolia) is one of major fruit tree in the family Rosaceae and have been bred for fruit production. To promote the development of breeding strategies and molecular researches for Japanese pear, we performed sequencing of transcripts in Japanese pear ‘Hosui’. For the purpose of exhaustively collecting information of expressed gene, the sequencings derived from various tissues and stages of Japanese pear were conducted by three methods, SMRT sequencing (PacBio), 454 pyrosequencing, and Sanger sequencing. With all reads obtained from these sequencing methods, we determined the comprehensive reference sequences of Japanese pear. Proteins were predicted and assigned the functional annotations. Finally, we developed a web-database 'TRANSNAP' (http://plantomics.mind.meiji.ac.jp/nashi/), which is the first public web resource of omics information in Japanese pear. This database provides the high reliable information in Japanese pear via user friendly web interfaces: the reference sequences, gene functional annotations by Gene Ontology, KEGG, and InterPro, and gene expression profiles from microarray experiments. In addition, by sequence comparisons with BLASTP searches, we provide protein sequences in Chinese and European pear having sequence similarities with Japanese pear proteins. On the bases of the information from sequence comparisons, users quickly and efficiently identify species-specific genes in Japanese pear. The database TRANSNAP has advantage for molecular research and breeding in Japanese pear, and the providing information will play a role for comparative analysis among other pear species or families.